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Estrogen Receptor Gene Expression in MCF-10A Cells Under Various Treatment and Culture Conditions

Journal: Cancer Cell International

Article Title: Morphologic transformation of human breast epithelial cells MCF-10A: dependence on an oxidative microenvironment and estrogen/epidermal growth factor receptors

doi: 10.1186/1475-2867-10-30

Figure Lengend Snippet: Estrogen Receptor Gene Expression in MCF-10A Cells Under Various Treatment and Culture Conditions

Article Snippet: Antibodies (Ab) and controls used were: ERα (62A3) mouse monoclonal Ab, EGFR rabbit polyclonal Ab, and anti-biotin Ab (Cell Signaling Technology; Beverly, MA); ERβ (PA1-313) rabbit Ab, human, recombinant ERα RP-310 and ERβ (long form) RP-312 (Affinity BioReagents; Golden, CO); EGF-stimulated A431 cell lysate (Upstate Biotechnology; Lake Placid, NY).

Techniques: Expressing